Paromomycin and Dihydrostreptomycin Binding to Escherichia coli Ribosomes

Binding of dihydrostreptomycin to Escherichia coli ribosomes: characteristics and equilibrium of the reaction.

The binding of dihydrostreptomycin to ribosomes and ribosomal subunits of a number of different Escherichia coli strains was studied, and the Mg(2+) and pH dependence, as well as the effect of salts and polynucleotides, was determined. The only requirement for binding with ribosomes and subunits from susceptible strains was 10 mm Mg(2+). Monovalent salts weakened the binding in a manner similar...

Binding of erythromycin to Escherichia coli ribosomes.

This paper concerns a precise study on the nature of the binding of erythromycin to Escherichia coli (Q13) ribosomes. (1) An equimolar amount of erythromycin bound to 50S ribosomal subunits at extremely low concentrations and this binding was not affected by the addition of 30S ribosomal subunits. (2) The binding of erythromycin to ribosomes was not significantly influenced by decreasing the Mg...

Further evidence of tetracycline binding to Escherichia coli ribosomes.

sucrose density gradients, indicated that tetracycline binds to 30S (Svedburg) ribosomal subunits (R. H. Connamacher and H. G. Mandel, Biochem. Biophys. Res. Commun. 20:98, 1965). Further analysis by the same technique demonstrated that the antibiotic binds not only to the 30S subunit but also to the 50S subunit of E. coli ribosomes (L. E. Day, J. Bacteriol 91:1917, 1966). J. A. Last, K. Izaki,...

Chloroplast and cytoplasmic ribosomes of Euglena: selective binding of dihydrostreptomycin to chloroplast ribosomes.

Dihydrostreptomycin binds preferentially to chloroplast ribosomes of wild-type Euglena gracilis Klebs var. bacillaris Pringsheim. The K(diss) for the wild-type chloroplast ribosome-dihydrostreptomycin complex is 2 x 10(-7) M, a value comparable with that found for the Escherichia coli ribosome-dihydrostreptomycin complex. Chloroplast ribosomes isolated from the streptomycin-resistant mutant Sm(...

The accumulation of "run-off" ribosomes during treatment of Escherichia coli with dihydrostreptomycin in vivo.